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Runx2 expression in bone is significantly decreased in Runx1f/fOsx-Cre mice. A, IHC staining RUNX2 antibody of femur paraffin sections from P0 Runx1f/fOsx-Cre (f/f/Δ) and WT (f/f) mice. B, quantification of A. C, ELISA detection of P1NP (pg/ml) in 3-month–old mouse serum indicating bone formation is affected by gene CKO. Results are expressed as mean ± S.D., n = 3 in each group. *, p < 0.05; **, p < 0.01; ***, p < 0.001.
