Figure 5.
The expression of Sox9, Ihh, Cyclin D1, and Ptc in the growth plate, and the expression of Ocn in femurs were significantly decreased in Runx1f/fOsx-Cre mice compared with WT mice. A–D, IF staining of the growth plates of P0 Runx1f/fOsx-Cre mice compared with that of WT mice to detect (A) Sox9, (B) Ihh, (C) Ptc, and (D) Cyclin D1. E, IF staining with anti-Osterix, and anti-Ocn antibodies of femur frozen sections from P0 Runx1f/fOsx-Cre (f/f/Δ) and WT (f/f) mice. F, quantification of A–E. Results are expressed as mean ± S.D., n = 3 in each group.