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. 2020 Jul 4;53(4):61–72. doi: 10.1267/ahc.20-00002

Fig. 3.

Fig. 3.

Effect of Ptpp-PDT on mitochondrial functions in NOZ cells. (A) The mitochondrial membrane potential was analyzed by flow cytometry using 4 μM JC-1 at 0 and 24 hr after Ptpp-PDT. (B) Western blot analysis of OXPHOS I (23 kDa), III (48 kDa) and V (55 kDa) in NOZ cells at 0, 6, 12 and 24 hr after Ptpp-PDT. Protein extracts (10 μg) were subjected to SDS-PAGE. (C) Protein bands were analyzed by densitometry and results are shown as a bar graph. Protein expression was normalized to β-actin (42 kDa). *P < 0.05, **P < 0.01, ***P < 0.001 vs. 0 hr for each protein. Data are shown as the mean ± SD of three independent experiments.