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. 2020 Jul 4;53(4):61–72. doi: 10.1267/ahc.20-00002

Fig. 6.

Fig. 6.

Effect of Ptpp-PDT on cytochrome c and cleaved caspase-3 in NOZ cells. Immunohistochemical localization of (A) cytochrome c and (B) cleaved caspase-3 at 0, 6, 12 and 24 hr after Ptpp-PDT. Negative controls are shown in the inset. Bar = 10 μm. (C) Quantitative analysis of cleaved caspase-3-positive cells. *P < 0.05, **P < 0.01 vs. 0 hr. Data are shown as the mean ± SD of three independent experiments. (D) Western blot analysis detected double bands for cleaved caspase-3 (17 and 19 kDa). Protein extracts (20 μg) were subjected to SDS-PAGE. β-actin (42 kDa) was used as a loading control.