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. 2020 Sep;190(9):1823–1832. doi: 10.1016/j.ajpath.2020.06.001

Figure 2.

Figure 2

Resolvin E1 (RvE1) counterregulation of leukotriene B4 (LTB4)–induced intracellular [Ca2+] ([Ca2+]i) increase is reversed by depletion of CMKLR1 in goblet cells. A and C: Cultured rat conjunctival goblet cells were transfected with Cmklr1 siRNA (100 nmol/L) or scrambled (sc) siRNA (100 nmol/L) for 48 hours or were not transfected. All cells were stimulated with LTB4 (10−8 mol/L) alone or with RvE1 (10−8 mol/L) 30 minutes followed by LTB4. A and B: Cells transfected with Cmklr1 siRNA or scsiRNA were stimulated with LTB4 or with RvE1 for 30 minutes followed by LTB4. The average [Ca2+]i level over time is shown in A, and the change in peak [Ca2+]i is shown in B. C and D: Similar experiments were performed with histamine dimaleate (H1, 10−5 mol/L) replacing LTB4. Arrows indicate addition of LTB4 or histamine. One-way analysis of variance nonparametric with Dunn multiple comparisons was used. Data are expressed as individual data points from each animal and means ± SEM. n = 7 rats (B and D). ∗P < 0.05 versus basal; †P < 0.05.