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. 2020 Sep;190(9):1823–1832. doi: 10.1016/j.ajpath.2020.06.001

Figure 3.

Figure 3

Resolvin D1 (RvD1) counterregulation of leukotriene B4 (LTB4)–induced intracellular [Ca2+] ([Ca2+]i) increase is reversed by lipoxin A4 receptor/formyl peptide receptor ALX/FPR2 antagonist BOC2 in goblet cells. A and B: Cultured rat conjunctival goblet cells were stimulated with either of the following treatments: LTB4 (10−8 mol/L) alone; RvD1 (10−8 mol/L) for 30 minutes followed by LTB4; BOC2 (10−4 mol/L) for 15 minutes followed by LTB4; or BOC2 for 15 minutes, followed by RvD1 for 30 minutes followed by LTB4. The average [Ca2+]i level over time is shown (A). B: Change in peak [Ca2+]i was calculated and is shown. C and D: Similar experiments were performed with histamine dimaleate (H1, 10−5 mol/L) replacing LTB4. Arrows indicate addition of LTB4 or H1. One-way analysis of variance nonparametric with Dunn multiple comparisons was used. Data are expressed as individual data points from each animal and means ± SEM. n = 6 rats (B and D). ∗P < 0.05 versus basal; †P < 0.05 versus LTB4 or histamine alone.