Figure 5.

Resolvin E1 (RvE1) and resolvin D1 (RvD1) use the same cellular Ca²⁺ stores as leukotriene B4 (LTB4) to increase the intracellular [Ca²⁺] ([Ca²⁺]_i) in goblet cells. Cultured rat goblet cells were transferred into Ca²⁺-free or normal KRB buffer for 2 hours or IP3R inhibitor 2-APB (10⁻⁶ mol/L) or vehicle was added for 15 minutes followed by RvE1 (A and B), RvD1 (C and D), or LTB4 (E and F), each at 10⁻⁸ mol/L. A, C, and E: The average [Ca²⁺]_i level over time is shown. B, D, and F: Change in peak [Ca²⁺]_i was calculated and is shown. Arrows indicate addition of RvE1 (A), RvD1 (C), or LTB4 (E). One-way analysis of variance nonparametric with Dunn multiple comparisons was used. Data are expressed as individual data points from each animal and means ± SEM. n = 7 rats (B); n = 6 rats (D and F). ^∗P < 0.05 versus basal; ^†P < 0.05 versus agonist alone.