Figure 3.

Identification of Ribosome-Bound mRNAs Enriched in PAPs from the Dorsal Hippocampus

(A) Flowchart for the TRAP and analysis of ribosome-bound mRNAs in astrocytes from the dorsal hippocampus. Sequencing raw data are in Table S1.

(B) Purity heatmap of the RNA-seq data for a selection of mRNAs specific for each type of brain cell. The centered value represents mRNAs with log₂(500 normalized reads), which corresponds to our threshold for the presence of an mRNA. Each column represents an independent cDNA library (n = 3 libraries).

(C) Gene Ontology analysis of mRNAs enriched in PAPs, relative to whole astrocytes (p ≤ 0.05, log₂ fold change [FC] ≤ −1).

(D) Raw sequencing data for a selection of the most PAP-enriched mRNAs sorted by log₂ fold change (p ≤ 0.05, log₂ fold change ≤ −1).