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. 2020 Aug 24;34:2058738420946192. doi: 10.1177/2058738420946192

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — FOXA1 is a pioneer factor mediating ARPE-19-specific expression of ENACα (a) ARPE-19 cells were transfected with non-specific siRNA (siNS) or two siRNAs targeting different sites of the FOXA1 mRNA (siFOXA1(1) and siFOXA1(2)) to knock down FOXA1 (left panel). Cells were treated with Dex (100 nM) for 8 or 24 h before being harvested, and ENACα expression in ARPE-19 cells was assessed by RT-qPCR. Total RNA was analyzed by RT-qPCR. All mRNA levels were normalized to that of 18S rRNA (right panel). (b) The expression of ENACα in AP-1-depleted ARPE-19 cells (left panel) was assessed by RT-qPCR. Total RNA was analyzed by RT-qPCR. All mRNA levels were normalized to that of 18S rRNA (right panel).

n.s.: not significant.

*P < 0.05.