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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Nat Med. 2020 Jun 29;26(8):1240–1246. doi: 10.1038/s41591-020-0937-x

Extended Data Fig. 5 |. AuC calculation using wile-type and mutant stalk-only construct.

Extended Data Fig. 5 |

Raw ELISA data used to generate Fig. 3j are shown. Stalk-only constructs with or without the A388V mutation (See Extended Data Fig. 7b for the amino acid sequence) were used to measure changes in the level of antibodies recognizing the wild-type (A388) of mutant (V388) stalk in human serum. twenty-nine pre-challenge serum samples from the influenza human challenge study participants were serially diluted and used for the ELISA. the AUC was calculated using graphPad Prism8 (v.8.3.0) with the baseline value of 0.1 (approximately 2 times the OD492 value from the control wells). Blue lines and numbers show the ELISA data and the AUC, respectively, obtained using the wild-type (A388) stalk construct. Red lines and numbers show the ELISA data and the AUC, respectively, obtained using the mutant (V388) stalk construct. graphs show mean and standard deviations from three independent measurements.