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. Author manuscript; available in PMC: 2020 Aug 27.
Published in final edited form as: Biochemistry. 2019 Oct 28;58(44):4447–4455. doi: 10.1021/acs.biochem.9b00805

Figure 10.

Figure 10.

GSK126 enhances only single-stranded DNA breaks mediated by topoisomerase IV. The top panel shows the enhancement of B. anthracis topoisomerase IV-mediated single- (purple) or double-stranded (black) DNA breaks at 10 min (filled bar) or 60 min (empty bar) in the absence or presence of 10 μM or 200 μM GSK126. The bottom panel shows the enhancement of E. coli topoisomerase IV-mediated single- (blue) or double-stranded (black) DNA breaks at 10 min (filled bar) or 60 min (empty bar) in the absence or presence of 10 μM or 200 μM GSK126. Error bars represent the SD of at least 3 independent experiments. Representative agarose gels are shown in Figure S8.