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. Author manuscript; available in PMC: 2020 Aug 27.
Published in final edited form as: Biochemistry. 2019 Oct 28;58(44):4447–4455. doi: 10.1021/acs.biochem.9b00805

Figure 3.

Figure 3.

GSK126 inhibits DNA supercoiling catalyzed by wild-type (WT) and fluoroquinolone-resistant gyrase. The effects of GSK126 on the supercoiling of relaxed DNA by WT (filled circles) and fluoroquinolone-resistant (empty circles) B. anthracis WT and GyrAS85L (top left, red), E. coli WT and GyrAS83L (top middle, green), and M. tuberculosis WT and GyrAA90V (top left, orange) gyrase are shown. Error bars represent the SD of at least three independent experiments. The bottom panel shows a table of IC50 values. Representative agarose gels are shown in Figure S1.