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. Author manuscript; available in PMC: 2020 Aug 27.
Published in final edited form as: Biochemistry. 2019 Oct 28;58(44):4447–4455. doi: 10.1021/acs.biochem.9b00805

Figure 5.

Figure 5.

GSK126 enhances only single-stranded DNA breaks mediated by gyrase. The top panel shows the enhancement of B. anthracis gyrase-mediated single- (red) or double-stranded (black) DNA breaks at 30 min (filled bar) or 3 h (empty bar) in the absence or presence of 10 μM or 200 μM GSK126. The middle panel shows the enhancement of E. coli gyrase-mediated single- (green) or double-stranded (black) DNA breaks at 10 min (filled bar) or 60 min (empty bar) in the absence or presence of 10 μM or 200 μM GSK126. The bottom panel shows the enhancement of M. tuberculosis gyrase-mediated single- (orange) or double-stranded (black) DNA breaks at 10 min (filled bar) or 60 min (empty bar) in the absence or presence of 10 μM or 200 μM GSK126. Error bars represent the SD of at least 3 independent experiments. Representative agarose gels are shown in Figure S3.