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. Author manuscript; available in PMC: 2020 Aug 27.
Published in final edited form as: Cell Rep. 2020 Aug 4;32(5):107997. doi: 10.1016/j.celrep.2020.107997

Figure 6. Knockdown of RGS6 in adult-born new neurons abolishes running effect on new neuron maturation.

Figure 6.

(A) A schematic illustration of retroviral vector expressing shRNA and GFP for targeting adult new neurons.

(B) Experimental timeline for accessing the effect of RGS6 knockdown on dendritic morphology of adult new neurons in running and sedentary mice.

(C) Sample confocal images of virus-infected and GFP+ neurons. Scale bar, 50μm.

(D) Sample traces of retrovirus shNC-infected neurons in running and sedentary mice.

(E) Running increases dendritic complexity of adult-born new neurons in mice injected with control shNC virus (F1,72=9.241, p < 0.01, MANOVA).

(F) Running increases the dendritic length of adult-born new neurons (p<0.01, t-test).

(G-I) RGS6 knockdown abolishes running-induced increased dendritic complexity (shRgs6-sedentary vs. shRgs6-running, F1,80 =0.23, p=0.633, MANOVA) and increased dendritic length (p>0.05, t-test).

(J) Experimental timeline for patch clamp recording of adult-born new neurons with RGS6 knockdown.

(K) Representative traces of mEPSCs recorded from GFP+ DG neurons in acute hippocampal slices derived from shNC and shRgs6 mice 3 weeks after retroviral injection.

(L-M) Cumulative probability distribution (shNC, n= 23 cells; shRgs6, n= 19 cells. t-test) and average frequency (P=0.0259, t-test). *P< 0.05, **P< 0.01, ***P< 0.001.