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. Author manuscript; available in PMC: 2020 Aug 27.
Published in final edited form as: Cell Rep. 2020 Aug 4;32(5):107997. doi: 10.1016/j.celrep.2020.107997

Figure 7. RGS6 is Required for Running-induced Learning and Memory Enhancement and Anxiolytic Effect.

Figure 7.

(A) Experimental timeline for assessing the effect of RGS6 knockdown on anxiety, spatial learning and memory in running and sedentary mice.

(B) A scheme cartoon illustrates elevated plus maze (EPM) test.

(C) Running mice exhibit reduced anxiety compared to sedentary mice as measured using an EPM test (P= 0.0141, Sedentary: 35.22 ± 6.25; Running: 63.02 ± 8.21).

(D) Knockdown of Rgs6 abolishes running-induced reduced anxiety in running mice (P= 0.0001, shNC: 87.60 ± 11.46; shRgs6: 23.51 ± 4.73).

(E) A scheme cartoon illustrates novel location test (NLT).

(F) Running mice exhibit improved spatial learning compared to sedentary mice measured by NLT (P= 0.0485, Sedentary: 12.07 ± 11.38; Running: 43.08 ± 9.233).

(G) Knockdown of RGS6 abolishes running-induced improvement on spatial learning in running mice (P= 0.0007, shNC: 51.46 ± 8.36; shRgs6: 7.27 ± 6.27).

(H) A scheme cartoon illustrates contextual fear conditioning test.

(I) Running mice exhibit improved contextual learning compared to sedentary mice measured by a contextual fear conditioning test (P= 0.014, Sedentary: 16.35 ± 5.05; Running: 35.54 ± 4.93).

(J) RGS6 Knockdown abolishes running-induced improvement on contextual learning in running mice (P= 0.0021, shNC: 45.01 ± 5.85; shRgs6: 18.61 ± 4.04). *P< 0.05, **P< 0.01, ***P< 0.001. Data are presented as mean ± s.e.m.