KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-RGS6 | antibodies-online Inc | #ABIN634403 |
| Mouse anti-HA.11 | Biolegend | # MMS-101R |
| Rabbit anti-MCM2 | Cell Signaling Technology | #4007 |
| Goat anti-DCX | Santa Cruz Biotechnology | # SC-8066 |
| Rabbit anti-GFAP | DAKO | #Z0334 |
| Rabbit anti-NeuN | Cell Signaling Technology | #4007 |
| Rabbit anti-DsRed | Takara | #632496 |
| Donkey anti-mouse 488 | Invitrogen | A21202 |
| Donkey anti-mouse 568 | Invitrogen | A10037 |
| Donkey anti-rabbit 488 | Invitrogen | A21206 |
| Donkey anti-rabbit 647 | Invitrogen | A31573 |
| Donkey anti-goat 568 | Invitrogen | A11057 |
| Goat anti-mouse 488 | Invitrogen | A11029 |
| Goat anti-rabbit 568 | Invitrogen | A11036 |
| Goat anti-rabbit 568 | Invitrogen | A11011 |
| Bacterial and Virus Strains | ||
| Lentivirus-STOP-RGS6-GFP | This paper | N/A |
| Lentivirus-STOP-GFP | This paper | N/A |
| Retrovirus-shRgs6-GFP | This paper | N/A |
| Retrovirus-shNC-GFP | (Guo et al., 2015) | N/A |
| Deposited Data | ||
| Source data associated with RiboTag sequencing | This paper | GEO: GSE142678 |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6J | Jackson Laboratory | RRID:IMSR_JAX:000664 |
| Mouse: Tg(Nestin-cre/ERT2) | Jackson Laboratory | RRID:IMSR_JAX:016261 |
| Mouse: RPL22HA/WT | Jackson Laboratory | RRID:IMSR_JAX:029977 |
| Mouse: Ascl1tm1(Cre/ERT2)Jejo/J | Jackson Laboratory | RRID:IMSR_JAX:012882 |
| Mouse: B6;129S6-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J | Jackson Laboratory | RRID:IMSR_JAX:007914 |
| Mouse: Nestin-GFP | Yamaguchi et al., 2000) | (Yamaguchi et al., 2000) |
| Oligonucleotides | ||
| Rgs6 Forward: GGATCGCTGATCCAGAAGAG | This study | N/A |
| Rgs6 Reverse: GGGGATTTTGGAGAGAAAGC | This study | N/A |
| Pgk1Forward: CTCCGCTTTCATGTAGAGGAAG | This study | N/A |
| Pgk1Reverse: GACATCTCCTAGTTTGGACAGTG | This study | N/A |
| Recombinant DNA | ||
| Retro-CAG-GFP-shRgs6 | This study | N/A |
| Retro-shNC | (Gao et al., 2015) | N/A |
| Retro-CAG-RFP | (Zhao et al., 2006) | N/A |
| Lenti-GFP-Control | This study | N/A |
| Lenti-GFP-RGS6 | This study | N/A |
| Software and Algorithms | ||
| Prism | GraphPad | https://www.graphpad.com; RRID:SCR_002798 |
| ImageJ | National Institute of Health | https://imagej.nih.gov/ij/docs/guide/user-guide.pdf |
| ANYmaze | Stoelting | https://www.stoeltingco.com/anymaze.html |
| RStudio | https://rstudio.com/ | |
| WGCNA | (Langfelder and Horvath, 2008) | |
| g:Profiler | https://biit.cs.ut.ee/gprofiler/gost | |
| Monocle | (Trapnell et al., 2014) | |