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. 2020 Aug 13;5(33):21241–21249. doi: 10.1021/acsomega.0c03097

Figure 2.

Figure 2

(a) Absorbance titration spectra of probe 5 (5 μM) in ethanol/4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, pH 7.4, 1/1, v/v) at 25 °C with gradual addition of 0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.5, and 2.0 equiv of Cu2+ ions. (b) Fluorescence titration spectra of probe 5 (5 μM) in ethanol/HEPES (pH 7.4, 1/1, v/v) at 25 °C with gradual addition of 0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.5, and 2.0 equiv of Cu2+ ions with an excitation of 460 nm.