Figure 13.

Stack plot of the ¹³C NMR (125.77 MHz) spectra of 2-deoxystreptamine at a range of pD (dilution 0.631–0.369 M from pD 2.28 to 12.00, hence the observed decrease in the signal intensity), measured relative to TMSP in 99.97% D₂O at 25 °C; C-1/3, C-2, C-4/6, and C-5 marked with the arrows. Using a relaxation delay (d1) of 2s and 512 transients, the acquisition time was ∼30 min for each spectrum.