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NMR titration curves for the (A) 1H, (B) 13C, and (C) 15N HMBC chemical shifts of 1.315–0.822 M kanamycin in 99.97% D2O at 25 °C and (D) pKa values of individual nitrogen atoms of kanamycin B determined using 1H (red), 13C (blue), and 15N HMBC (green) NMR spectroscopy.
