. 2020 Aug 11;5(33):21094–21103. doi: 10.1021/acsomega.0c02744

Table 5. pK_a Values of the Individual Nitrogen Atoms of Netilmicin Determined Using ¹H, ¹³C, and ¹⁵N HMBC NMR Spectroscopy in This Work and Then Compared With the Published Data, as Indicated.

	individual nitrogen atoms pK_a
method	N-1	N-3	N-2′	N-6′	N-3″
¹H NMR^a	8.15	6.55	8.10	9.27^b	8.48
¹³C NMR^a	8.11	6.50	8.11	9.32	8.50
¹⁵N HMBC NMR^a	8.20	6.51	8.23	9.37	8.45

This work. As far as can be determined, there are no literature data for the pK_a values of netilmicin.

The pK_a value of N-6′ of netilmicin determined using ¹H NMR spectroscopy (in this work) is the average pK_a of the values of N-6′ obtained using ¹H NMR spectroscopic data for 6′a (9.29) and 6′b (9.25).

Table 5. pKa Values of the Individual Nitrogen Atoms of Netilmicin Determined Using 1H, 13C, and 15N HMBC NMR Spectroscopy in This Work and Then Compared With the Published Data, as Indicated.

Table 5. pK_a Values of the Individual Nitrogen Atoms of Netilmicin Determined Using ¹H, ¹³C, and ¹⁵N HMBC NMR Spectroscopy in This Work and Then Compared With the Published Data, as Indicated.