. 2020 Aug 11;5(33):21094–21103. doi: 10.1021/acsomega.0c02744

Table 6. Average pK_a Values of the Individual Nitrogen Atoms of the Indicated Aminoglycosides Determined Using ¹H, ¹³C, and ¹⁵N HMBC NMR Spectroscopy.

	individual nitrogen atoms pK_a
aminoglycoside	N-1	N-3	N-2′	N-6′	N-3″	N-4‴
tobramycin	7.55	6.70	7.75	9.10	7.68
kanamycin B	8.10	6.78	7.36	8.97	7.65
amikacin		7.64		8.81	8.05	9.89
sisomicin	7.42	6.22	8.00	9.30	8.50
netilmicin	8.15	6.52	8.14	9.29	8.47

Table 6. Average pKa Values of the Individual Nitrogen Atoms of the Indicated Aminoglycosides Determined Using 1H, 13C, and 15N HMBC NMR Spectroscopy.