Fig. 3.

Elution curves for the final optimized separation method on a multi-element standard (A) and a human plasma sample (B). The standard contains the elements listed in Fig. 1A with mass of 100 ng in 0.5 mL, and the plasma sample contains 114,628 ng Na, 794 ng Mg, 1112 ng Ca, 7298 ng P, 107295 ng S, 64 ng Cu, 33 ng Fe, and 46 ng Zn. The results of Ti, Mn, Cr, Ga, and Cd of the plasma are not shown because their masses in the sample were lower than 1 ng and could therefore not be measured accurately