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. Author manuscript; available in PMC: 2020 Aug 27.
Published in final edited form as: Anal Bioanal Chem. 2018 Nov 22;411(3):765–776. doi: 10.1007/s00216-018-1498-4

Table 1.

Protocol for separating Cu, Fe, Zn, and Cd from sample matrix for MC-ICPMS isotopic analysis

Step Reagent Volume Collection
Loading AG-MP1 resin 0.25 mL
 Cleaning resin 2 M HNO3 2 mL
 Rinsing Milli-Q 0.5 mL
 Rinsing 1 M HCl 0.5 mL
 Conditioning 11 M CH3COOH + 4 M HCl + 0.01% H2O2 0.4 mL × 2
 Sample loading Sample (in 11 M CH3COOH + 4 M HCl + 0.01% H2O2) 0.5–1 mL Na, Mg, Ca, S, P
 Matrix removal 11 M CH3COOH + 4 M HCl + 0.01% H2O2 0.5 mL × 10 Na, Mg, Ca, S, P
 Cu elution 5 M HCl + 0.01% H2O2 0.4 mL × 16 Cu
 Fe elution 1 M HCl 0.2 mL × 14 Fe
 Zn elution 2 M HNO3 + 0.1 M HBr 0.2 mL × 12 Zn
 Cd elution 2 M HNO3 0.2 mL × 10 Cd