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Cooperative-binding split aptamer (CBSA)-based assay for small-molecule detection. (A) In the absence of target, both CBSA fragments remain separate, and the fluorophore on the labeled strand is quenched due to close proximity to a quencher. (B) One target binding assembles both CBSA fragments which (C) promotes cooperative binding of a second target, separating fluorophore and quencher and thus producing an increase in fluorescence.
