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Protection from PCV2 strain DF-1 challenge in mice. All of the mice were challenged with 100 μL of 106.5 TCID50/mL of the PCV2 strain DF-1 at 56 dpi and examined for 28 days. Spleens were isolated and the genomes were extracted to measure the content of PCV2 using quantitative real-time PCR. Date are shown as mean ± SEM, statistical differences between each group was measured by one-way ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001, ns represented not significant
