Fig. 2.
Effect of sodium salicylate (NaSa) on siderophore and pyocyanin production in P. aeruginosa PAO1. (a) Growth curves of PAO1 with increasing concentrations of NaSa (0–10 mM) in 50 % serum in AB medium. (b) Visual appearance of supernatants. Effect of NaSa (0–10 mM) and serum (0 and 50 %) on (c) siderophores and (d) pyocyanin production after 3 days of growth. Note: 100% corresponds to OD 6.85 and 1.91 for siderophores and pyocyanin, respectively. Each bar represents the average data from three independent experiments. Error bars represent ±sd. * Indicates a statistically significant difference compared to the untreated 0 mM NaSa group (in the 0 and 50% serum groups, respectively) with a P-value <0.05 based on one-way ANOVA Dunnett's post hoc test.