Table 2.
Characterization of clinical P. aeruginosa strains from chronic wounds according to their production of the QS signals C4-HSL, 3-OC12-HSL and PQS and biofilm formation on polystyrene. Supernatants from 24-h-old cultures of each strain in 50 % serum in saline were used for QS-signal analysis using fluorescent reporter strains. Biofilm formation was evaluated on polystyrene surfaces after 48 h culture in 50 % serum in saline using the Calgary biofilm device
|
Strain |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
PAO1 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
C4-HSL |
+ |
− |
− |
+ |
+ |
+ |
− |
− |
+ |
− |
+ |
+ |
+ |
− |
+ |
|
3-OC12-HSL |
+ |
− |
− |
+ |
+ |
+ |
+ |
− |
− |
− |
+ |
+ |
+ |
− |
+ |
|
PQS |
+ |
− |
− |
+ |
+ |
+ |
− |
− |
+ |
− |
+ |
+ |
− |
− |
+ |
|
Biofilm OD520 (STDEV) |
1.2 (0.1) |
1.4 (0.4) |
1.1 (0.6) |
1.8 (0.2) |
2.4 (0.2) |
2.0 (0.7) |
1.6 (0.9) |
1.1 (0.5) |
1.2 (0.5) |
1.0 (0.4) |
0.9 (0.1) |
0.9 (0.4) |
1.0 (0.2) |
0.8 (0.2) |
2.0 (0.9) |