Table 3.

List of strains used in this study

Strain name	Characteristic	Reference
From 1 to 14	Clinical wound isolates of P. aeruginosa	[96]
PAO1	Reference strain of P. aeruginosa, Pseudomonas Genetic Stock Center (http:// www.pseudomonas.med.ecu.edu, strain PAO0001)
MH155	3-OC12-HSL signal reporter. Escherichia coli MT102 with lasB::gfp(ASV)	[34]
MH2O5	C4-HSL signal reporter. E. coli MT102 with ahyR-ahyI::gfp(ASV)	[91]
∆pqsC PpqsA::gfp(ASV)	PQS signal reporter. PAO1 ∆pqsC with pqsA::gfp(ASV)	[97]
PAO1 PlasB::gfp(ASV)	las reporter. PAO1 with a mini-Tn5 insert containing a translational fusion of the LasR-regulated lasB promoter and a gene coding for an unstable version of green fluorescent protein (GFP), gfp(ASV)	[34]
PAO1 rhlA::gfp(ASV)	rhl reporter. PAO1 carrying a pMHRA plasmid containing RhlR-regulated rhlA::gfp(ASV) translational fusion inserted into the vector pMH391	[30]
PAO1 pqsA::gfp	pqs reporter. PAO1 carrying the plasmid pAC37 containing a transcriptional fusion of the pqs-regulated pqsA promoter and gfp(ASV)	[97]
MH694	PAO1 ∆lasI. QS-deficient mutant with a deletion of lasI gene	[98]
MH698	PAO1 ∆rhlI. QS-deficient mutant with a deletion of rhlI gene	[35]
∆pqsC	PAO1 ∆pqsC. QS-deficient mutant with a deletion of pqsC gene	[99]