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. Author manuscript; available in PMC: 2020 Aug 27.
Published in final edited form as: Cancer Res. 2019 Aug 20;79(19):4896–4910. doi: 10.1158/0008-5472.CAN-19-0020

Figure 3. miR-571 and APC/C are two concerted pathways to regulate Geminin expression and DNA re-replication.

Figure 3.

(A and B) HCT 116 cells (A) and U2OS cells (B) transfected with indicated siRNAs, miRNAs or both. 48 hr after transfection, Geminin protein levels were detected by Western blot (left panel) and DNA re-replication (right panel) were analyzed by FACS. (C) Quantification results of HCT-116 and U2OS cells treated in Fig. 3A and 3B (Data represent mean ± SE from three independent experiments, **p≤0.01). (D and E) HCT 116 cells (D) and U2OS cells (E) transfected with indicated siRNAs, miRNA inhibitors or both. 48 hr after transfection, Geminin protein levels (left panel) and DNA re-replication (right panel) were analyzed. (F) Quantification results of HCT-116 and U2OS cells treated in Fig. 3D and 3E (Data represent mean ± SE from three independent experiments, *p≤0.05, **p≤0.01).