KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-RPS28 | Aviva systems biology | Cat# ARP65601_P050 |
| Rabbit polyclonal anti-RPS7 | Bethyl laboratories | Cat# A300–740A; RRID:AB_533451 |
| Rabbit polyclonal anti-RPL35A | Bethyl laboratories | Cat# A305–106A; RRID:AB_2631501 |
| Mouse monoclonal anti-GAPDH | Thermo Fisher | Cat# AM4300; RRID:AB_437392 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Cycloheximide | Sigma-Aldrich | Cat# C4859–1ML |
| Sodium arsenite | Sigma-Aldrich | Cat# 1062771000 |
| Critical Commercial Assays | ||
| Cell 96 Aqueous One Solution Cell Proliferation Assay (MTS) | Promega | Cat# G3582 |
| Lipofectamine 2000 | Thermo Fisher | Cat# 11668019 |
| Superscript IV RT kit | Thermo Fisher | Cat# 12594100 |
| TRIZOL reagent | Thermo Fisher | Cat# 15596026 |
| Experimental Models: Cell Lines | ||
| Mouse: Hepa 1–6 cells | ATCC | CRL-1830 |
| Human: HeLa cells | ATCC | CCL-2 |
| Oligonucleotides | ||
| Anti-control: GtaCgCgGaaTaCTtC | Exiqon | N/A |
| Anti-Leu3ťs: tGTcAGgAgTggGaT | Exiqon | N/A |
| Anti-Leu3ťsMM: tCTcACgAgTggGaT | Exiqon | N/A |
| Anti-Leu3ťsMM2: tGTcAAgAcTggGaT | Exiqon | N/A |
| Northern probe for LeuCAG3ťsRNA: 5'-gtgtcagg agtgggattcg-3' | IDT | N/A |
| Northern probe for mouse ITS1: 5'-acgccgccgct cctccacagtctcccgtt-3' | IDT | N/A |
| Northern probe for mRNAs: See Table S3 | IDT | N/A |
| Oligonucleotides for site-directed mutagenesis: See Table S4 | IDT | N/A |
| Recombinant DNA | ||
| CMV promoter-mouseRPS28 wt | This paper | N/A |
| CMV promoter-mouseRPS28 target-A mutant | This paper | N/A |
| CMV promoter-mouseRPS28 target-B mutant | This paper | N/A |
| CMV promoter-mouseRPS28 non-target mutant | This paper | N/A |
| Software and Algorithms | ||
| RNA-hybrid program | Krüger and Rehmsmeier, 2006 | https://bibiserv.cebitec.uni-bielefeld.de/rnahybrid |
| Mega software | Kumar et al., 2016b | https://www.megasoftware.net |
| MegAlign program | Burland, 2000 |
https://www.dnastar.com/software/ molecular-biology/ |
| PRISM 8.0 | N/A | https://www.graphpad.com/ |
| Integrative genomics viewer | Robinson et al., 2011 | https://software.broadinstitute.org/software/igv/ |
| Clustal Omega | Sievers et al., 2011 | https://www.ebi.ac.uk/Tools/msa/clustalo/ |
| TopHat2 (v.2.0.14) | Trapnell et al., 2009 | https://ccb.jhu.edu/software/tophat/index.shtml |