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. 2020 Aug 27;19:170. doi: 10.1186/s12934-020-01427-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Cell cultivation and lysis within microcapsules. a Microscopy images of HC-CAMs before and after cell growth. The scale bar corresponds to 200 µm. b Growth kinetics of HC-CAMs-encapsulated E. coli cells as a function of time. Data were fitted using the Gompertz equation. c Lysis efficiency of E. coli cells encapsulated in HC-CAMs was determined by cell viability upon treatment with increasing BugBuster concentration. d BugBuster induced lysis efficiency of encapsulated E. coli in microcapsules with increasing number of PSS-PAH layers