Fig. 5.

HC-PCAMs behave as individual reaction compartments that can be distinguished based on enzymatic activity. a Microscopic (left side) and COPAS analysis (right side) of mixtures of HC-PCAMs (1:5 ratio of microcapsules with cells expressing Gly-SrtF wild type:Gly-SrtF-C195G) after 21 h’ reaction with 50 µM FITC-LPQTGE. Consistent with the mixing ratio, the analysis yielded two well-separated microcapsule populations. Microcapsules expressing the wild type enzyme were clearly distinguishable from those expressing the inactive mutant. b Unlike HC-PCAMs, non-coated microcapsules exchanged cell lysates and reaction products and yield a single population with an intermediate green fluorescence intensity