Figure 1.

Antigen Valencies Influence Cognate B Cell Stimulation In Vivo within Hours

(A) To-scale models of eOD-GT5 constructs: eOD (green), CD4bs (yellow), scaffold (cyan), and glycans (slate).

(B–D) Intravital imaging of iLNs 2–3.5 h after immunization with eOD-GT5 antigens. (B) Schematic. (C) Speed of VRC01^gHL B cells normalized to polyclonal B cells. (D) Stationary VRC01^gHL B cells (speed <3 μm/min).

Data in (A)–(D) are from three experiments with n = 2–5 mice/group. Bars represent the mean.

(E–H) VRC01^gHL B cell activation at 24 h. (E) Activation markers on VRC01^gHL B cells. (F) Quantification of activation markers in (E), geometric mean fluorescence intensity (MFI). (G) VRC01^gHL B cell size. (H) Quantification of VRC01^gHL B cell size (mean FSC-A). Data in (E)–(H) are from two experiments, with n = 6 mice. Bars represent the mean.

^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001; ^∗∗∗∗p < 0.0001. See also Figures S1 and S2, Video S1, and Table S1.