Figure 2.

Differential Effects of Antigen Valencies on B Cell Division In Vivo

(A–C) Proliferative responses of VRC01^gHL B cells 3 days after immunizations. (A) Division profiles. The gates show CTV^hi (undivided) and CTV^lo (divided) cells. (B) Divided VRC01^gHL B cell number. Bars represent the geometric mean. (C) Undivided VRC01^gHL B cells as a frequency of total B cells, normalized to the unimmunized control. Bars represent the mean. Data in (A)–(C) are from two experiments with n = 6 mice.

(D–F) VRC01^gHL B cell activation and localization patterns 24 h after immunizations. (D) Large (FSC-A^hi) CCR7^hi VRC01^gHL B cells. (E) Representative iLNs showing VRC01^gHL B cells (CTV, white), B cell zones (IgD, blue), and T cell zones (CD4, green). CTV signals were digitally dilated. Scale bar, 300 μm. (F) Distribution of VRC01^gHL B cells. The total numbers of cells analyzed are indicated within bars. Data in (D)–(F) are from two experiments with n = 6 mice.

^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001; ^∗∗∗∗p < 0.0001.