| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-mouse/human Bcl6 AF647 (clone K112-91) | BD Bioscience | Cat# 561525; RRID:AB_10898007 |
| Rat monoclonal anti-mouse/human B220 BV785 (clone RA3-6B2) | Biolegend | Cat# 103246; RRID:AB_2563256 |
| Rat monoclonal anti-mouse CCR7 PE (clone 4B12) | Biolegend | Cat# 120106; RRID:AB_389358 |
| Rat monoclonal anti-mouse CD16/32 (clone 93) | Biolegend | Cat# 101302; RRID:AB_312801 |
| Rat monoclonal anti-mouse CD4 (clone GK1.5) | Bio X Cell | Cat# BE0003-1; RRID:AB_1107636 |
| Rat monoclonal anti-keyhole limpet hemocyanin (rat IgG2a isotype control; clone LTF-2) | Bio X Cell | Cat# BE0090; RRID:AB_1107780 |
| Rat monoclonal anti-mouse CD4 AF700 (clone RM4-4) | Biolegend | Cat# 116022; RRID:AB_2715958 |
| Rat monoclonal anti-mouse CD4 AF488 (clone RM4-5) | Biolegend | Cat# 100529; RRID:AB_389303 |
| Rat monoclonal anti-mouse/human CD44 PerCPCy5.5 (clone IM7) | Biolegend | Cat# 103032; RRID:AB_2076204 |
| Mouse monoclonal anti-mouse CD45.1 BV510 (clone A20) | Biolegend | Cat# 110741; RRID:AB_2563378 |
| Mouse monoclonal anti-mouse biotin (clone A20) | Biolegend | Cat# 110704; RRID:AB_313493 |
| Rat monoclonal anti-mouse CD69 PECy7 (clone H1.2F3) | Biolegend | Cat# 104512; RRID:AB_493564 |
| Rat monoclonal anti-mouse CD86 BV605 (clone GL-1) | Biolegend | Cat# 105037; RRID:AB_11204429 |
| Hamster monoclonal anti-mouse CD95 APC-R700 (clone Jo2) | BD Bioscience | Cat# 565130; RRID:AB_2739078 |
| Hamster monoclonal anti-mouse CD95 BV510 (clone Jo2) | BD Bioscience | Cat# 563646; RRID:AB_2738345 |
| Rat monoclonal anti-mouse CXCR5 biotin (clone L138D7) | Biolegend | Cat# 145510; RRID:AB_2562126 |
| Rat monoclonal anti-mouse/human GL7 PerCPCy5.5 (clone GL7) | Biolegend | Cat# 144610; RRID:AB_2562979 |
| Rat monoclonal anti-mouse/human GL7 PacificBlue (clone GL7) | Biolegend | Cat# 144614; RRID:AB_2563292 |
| Rat monoclonal anti-mouse IgD BV510 (clone 11-26c.2a) | Biolegend | Cat# 405723 RRID:AB_2562742 |
| Rat monoclonal anti-mouse AF700 (clone 11-26c.2a) | Biolegend | Cat# 405730; RRID:AB_2563341 |
| Rat monoclonal anti-mouse AF647 (clone 11-26c.2a) | Biolegend | Cat# 405708; RRID:AB_893528 |
| Rat monoclonal anti-mouse/human IRF4 PE | Biolegend | Cat# 646404; RRID:AB_2563005 |
| Rat monoclonal anti-mouse PD1 BV605 (clone 29F.1A12) | Biolegend | Cat# 135220; RRID:AB_2562616 |
| Rat monoclonal anti-mouse CD169 PE | Biolegend | Cat# 142404; RRID:AB_10915697 |
| Rabbit polyclonal anti-GFP AF488 | Thermo Fisher Scientific | Cat# A-21311; RRID:AB_221477 |
| Goat Anti-Mouse IgG, Human ads-HRP | Southern Biotech | Cat# 1030-05; RRID:AB_2619742 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| eOD-GT2gp61 60-mer | This manuscript | N/A |
| eOD-GT5gp61 60-mer | This manuscript | N/A |
| eOD-GT2gp61 4-mer | This manuscript | N/A |
| eOD-GT5gp61 4-mer | This manuscript | N/A |
| eOD-GT5gp61 4-mer (2B22) | This manuscript | N/A |
| eOD-GT5 4-mer | This manuscript | N/A |
| eOD-GT5 8-mer | This manuscript | N/A |
| eOD-GT2 60-mer | This manuscript | N/A |
| eOD-GT5 60-mer | This manuscript | N/A |
| eOD-GT5-KO2 60-mer | Jardine et al., 2013 | N/A |
| ISCOM-like saponin nanoparticle adjuvant | Cirelli et al., 2019 | N/A |
| Cholesterol | Avanti Polar Lipids | Cat# 700000 |
| 16:0 PC (DPPC) | Avanti Polar Lipids | Cat# 850355 |
| N-Decanoyl-N-methylglucamine (MEGA-10) | MilliporeSigma | Cat# D6277 |
| Quil-A saponin | InvivoGen | Cat# vac-quil |
| Avi-tagged eOD-GT5 | Jardine et al., 2013 | N/A |
| Avi-tagged eOD-GT2 | Jardine et al., 2013 | N/A |
| Avi-tagged eOD-GT5-KO2 | Jardine et al., 2013 | N/A |
| Streptavidin PECy7 | Biolegend | Cat# 405206 |
| Streptavidin APC | Biolegend | Cat# 405207 |
| Streptavidin PE | Biolegend | Cat# 405204 |
| Streptavidin Alexa Fluor 568 | Thermo Fisher Scientific | Cat# S11226 |
| Fixable Viability Dye eFluor 780 | Thermo Fisher Scientific | Cat# 65-0865-18 |
| 1-step Ultra TMB-ELISA substrate | Thermo Fisher Scientific | Cat# 34029 |
| Sodium phosphate monobasic (NaH2PO4) | MilliporeSigma | Cat# S0751 |
| Sodium phosphate dibasic (NH2HPO4) | MilliporeSigma | Cat# 71645 |
| 16% Paraformaldehyde Aqueous Solution, EM Grade | Electron Microscopy Science | Cat# 15710 |
| Ethylenediaminetetraacetic acid (EDTA), 0.5M | Thermo Fisher Scientific | Cat# AAJ15694AE |
| Pluronic F-127 | Thermo Fisher Scientific | Cat# P3000MP |
| Critical Commercial Assays | ||
| EasyStep Mouse CD4+ T Cell Isolation Kit | STEMCELL Technologies | Cat# 19852 |
| EasyEights EasySep Magnet | STEMCELL Technologies | Cat#18103 |
| QuadroMACS separator | Miltenyi Biotec | Cat# 130-091-051 |
| CellTrace Violet Cell Proliferation Kit | Thermo Fisher Scientific | Cat# C34557 |
| CellTracker Deep Red Dye | Thermo Fisher Scientific | Cat# C34565 |
| Alexa Fluor 647 Protein Labeling Kit | Thermo Fisher Scientific | Cat# A20173 |
| Alexa Fluor 488 Protein Labeling Kit | Thermo Fisher Scientific | Cat# A10235 |
| Pacific Blue Protein Labeling Kit | Thermo Fisher Scientific | Cat# P30012 |
| Bir-A biotin-protein ligase standard reaction kit | Avidity | Cat# BirA500 |
| CD43 (Ly-48) MicroBeads, mouse | Miltenyi Biotec | Cat# 130-049-801 |
| Cytofix Fixation Buffer | BD Bioscience | Cat# 554655 |
| eBioscience Foxp3/Transcription Factor Fixation/Permeabilization kit | Thermo Fisher Scientific | Cat# 00-5521-00 |
| Cholesterol Quantification Assay Kit | MilliporeSigma | Cat# CS0005-1KT |
| Sphero Blank Calibration Particles | BD Bioscience | Cat# 556296 |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6J mice | Jackson Laboratory | IMSR_JAX: 000664 |
| Mouse: VRC01gHL | Abbott et al., 2018 | N/A |
| Mouse: SMARTA | Oxenius et al., 1998 | N/A |
| Mouse: C57BL/6-Tg(UBC-GFP)30Scha/J (B6.GFP) | Jackson Laboratory | IMSR_JAX: 004353 |
| Mouse: B6.SJL-PtprcaPepcb/BoyJ | Jackson Laboratory | IMSR_JAX: 002014 |
| Mouse: B6.Cg-Tg(CAG-DsRed∗MST)1Nagy/J (DsRed) | Jackson Laboratory | IMSR_JAX: 006051 |
| Mouse: B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J (CFP) | Jackson Laboratory | IMSR_JAX: 004218 |
| Software and Algorithms | ||
| FlowJo 10 | FlowJo | https://www.flowjo.com/ |
| Adobe Illustrator CC 2020 | Adobe | https://www.adobe.com/ |
| Adobe After Effects CC 2020 | Adobe | https://www.adobe.com/ |
| ZEN system | Carl Zeiss | https://www.zeiss/ |
| Microsoft Office Excel | Microsoft | https://www.microsoft.com/ |
| Fiji/ImageJ | Open source | fiji.sc |
| Imaris 9.0.1+ | Bitplane | https://imaris.oxinst.com/ |
| Imaris XT | Bitplane | https://imaris.oxinst.com/ |
| RStudio v1.1+ | RStudio | https://rstudio.com/ |
| Spatstat (R package) | The R Foundation | https://www.r-project.org/ |
| FACSDiva | BD Bioscience | https://www.bdbioscience.com |
| The PyMOL Molecular Graphics System, Version 2.0 | Schrödinger | https://pymol.org/ |
| Prism 8 | GraphPad | https://www.graphpad.com/ |
| Other | ||
| 10K centrifugal filters | Amicon | Cat# UFC501096 |
| 50K centrifugal filters | Amicon | Cat# UFC505096 |
| PD-10 desalting columns | GE Life Sciences | Cat# 17-0851-01 |
| LS Columns | Miltenyi Biotec | Cat# 130-042-401 |
| Dulbecco’s phosphate-buffered saline (DPBS) with calcium and magnesium | Thermo Fisher Scientific | Cat# 14040133 |
| RPMI 1640 Medium | Thermo Fisher Scientific | Cat# 11875135 |
| PBS, 1X, pH 7.4 ± 0.1 | Corning | Cat# 21-040-CV |
| Ammonium-Chloride-Potassium (ACK) lysing buffer | Thermo Fisher Scientific | Cat# A1049201 |
| Sucrose | Thermo Fisher Scientific | Cat# S3-500 |
| Fetal Bovine Serum | Gemini | Cat# 100-106 |
| Bovine Serum Albumin | MilliporeSigma | Cat# A7906 |
| Normal Rat Serum | STEMCELL Technologies | Cat# 13551 |
| FACSCelesta | BD Bioscience | N/A |
| LSRFortessa | BD Bioscience | RRID:SCR_018655 |
| FACSAria III | BD Bioscience | RRID:SCR_016695 |
| LSM780 | Carl Zeiss | N/A |
| LSM880 with Airyscan | Carl Zeiss | N/A |
| SP8 multiphoton/confocal system | Leica Microsystems | N/A |
| 2104 EnVision Mulilabel Plate Reader | PerkinElmer | N/A |
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