Fig. 10.

Practical applications of SiMREPS. (A) Schematic depicting the use of SiMREPS for analysis of double-stranded DNA. (B) Standard curves from a SiMREPS assay for the cancer point mutation EGFR T790M using super-resolution and diffraction-limited analysis methods. (C) Comparison of accepted traces from low mutant allelic fraction (1:1 million) and wild-type-only conditions illustrating the high specificity of SiMREPS. (D) Schematic depiction of SiMREPS-based aptasensors for the detection of small molecules. (E) Standard curves and specificity of detection of different small molecules and spiked-in small molecule samples using SiMREPS-based aptasensors. (F) Schematic depicting the detection of telomerase activity on single substrate molecules. (G) Time traces of fluorescent probes in the presence and absence of telomerase. (H) High selectivity of the SiMREPS assay for the detection of telomerase. (I) Schematic of in situ detection of miRNAs using SiMREPS with nanoflare probes. (J) Kinetic behaviors of fluorescent probes undergoing specific binding to a single miRNA molecule and non-specific binding; (K) Quantification of the control experiments shown in (K) illustrating the importance of kinetic filtering. (A)-(C) Reprinted with permission from ref. [17]. Copyright 2018 Journal of the American Chemical Society. (D) and (E) Reprinted with permission from ref. [34]. Copyright 2019 Analytical Chemistry. (F)-(H) Reprinted with permission from ref. [57]. Copyright 2017 Analytical Chemistry. (I)-(K) Reprinted with permission from ref. [64]. Copyright 2019 Analytical Chemistry.