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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Trends Analyt Chem. 2019 Dec 4;123:115764. doi: 10.1016/j.trac.2019.115764

Fig. 2.

Fig. 2.

Kinetic fingerprinting with transient probing improves specificity. (A) The irreversible interaction between a fluorophore-labeled probe and a surface-bound target molecule is indistinguishable from nonspecific binding to spurious sequences on the surface, or to the surface itself. (B) The reversible interaction between a short fluorophore-labeled oligonucleotide probe and a surface bound target generates a kinetic fingerprint (fluorescence intensity versus time trace) that can be distinguished from nonspecific binding.