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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Trends Analyt Chem. 2019 Dec 4;123:115764. doi: 10.1016/j.trac.2019.115764

Fig. 5.

Fig. 5.

SiMREPS data acquisition. (A) FP-target binding and dissociation occurs over the observed time window and the resulting changes in fluorescent signal are observed via TIRFM. (B) A single-frame snapshot showing the locations of single bound FP molecules in a field of view under the microscope. (C) A molecule of target is present at this location, as confirmed by the repetitive binding-dissociation pattern of the FP that constitutes a kinetic fingerprint. (D) Nonspecific binding at other locations gives rise to a smaller number of binding-dissociation transitions and/or different kinetics that are easily distinguished so that only genuine target molecules are counted.