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. 2020 Jul 2;4:100029. doi: 10.1016/j.yjsbx.2020.100029

Fig. 1.

Fig. 1

EspB particle orientation is critical for cryoEM reconstruction. A) CryoEM micrograph of purified EspB (0.5 mg/mL) showing only EspB top views. B) CryoEM micrographs of purified EspB (5 mg/mL) supplemented with 0.05% fluorinated octyl maltoside showing different orientations of EspB suitable for cryoEM reconstruction. C) Top 2D classes obtained after the first round of 2D classification showing the different orientations obtained in the presence of a low detergent concentration.