Fig. 6.

C1 reconstruction shows residual extra-density corresponding to the C-terminal domain. A. CryoEM map of EspB when no symmetry operations are applied during reconstruction (C1 symmetry). The residual extra density is represented in red and corresponds to the C-terminal domain. B. Example of classes of projection obtained during the 2D classification shown in parallel with the corresponding orientation of the 3D map. Blurry density (white arrows) was identified close to the heptamer on projection with a 2D class orientation. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)