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Characterization of cortical layers present after 60 days in culture. A) Cajal-Retzius neurons stained for reelin show the presence of marginal zone. B) Staining for deep layer neurons (CTIP2) and neuronal markers (TUJ1). C) Upper-layer marker SATB2 indicate the presence of neurons belonging to the cortical layer IV. Immunostainings were repeated on four brain organoids from three independent experiments. Stitched images at 20×, scale bars: 500 μm.
