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. Author manuscript; available in PMC: 2020 Aug 27.
Published in final edited form as: HardwareX. 2019 Oct 11;6:e00084. doi: 10.1016/j.ohx.2019.e00084

Fig. 22.

Fig. 22.

Immunostaining for apoptosis marker Caspase 3. A) Cells expressing Nestin and cleaved caspase 3 (Cl Caspase-3) at day 60 B) and day 150 show no structural compromise of the cortical architecture. CTIP2 (layer 5) and CUX1 (layer 2) markers of cortical layers are included for this time point. Immunostainings were repeated on four brain organoids from three independent experiments for day 60 and four brain organoids from one independent experiment for day 150.Scale bars: 100 μm. (B) stitched image.