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. 2020 Apr 29;27(9):2280–2286. doi: 10.1016/j.sjbs.2020.04.039

Table 1.

Comparison between the effects of different concentrations from both SCR and TSA treatments on the cleavage blastocyst rates of SCNT sheep embryos.

No & type of treatment Concentration of treatment & No of ova No and % of cleavage rate Degenerating ova no & % Total cleavage % Total Blastula stage Total degenerating
Scripted SCR
179 ova
5 nM = 64 ova 11 = 17.18% 53 = 82.82% 41/179 = 22.91% 3/41 = 7.31%* 138/179 77.09%
50 nM = 73 ova 14 = 19.17% 59 = 80.82%
500 nM = 42 ova 16 = 38.09%* 26 = 61.91%
Trichostatin (A) TSA
134 ova
5 nM = 53 ova 12 = 22.64% 41 = 77.36% 35/134 = 28.69% 0/35 = 0% 87/134 = 71.31%
50 nM = 43 ova 8 = 18.6% 35 = 81.4%
500 nM = 38 ova 15 = 39.47%* 23 = 60.53%
Control 275 ova 0 nM = 94 ova 29 = 30.85%* 65 = 69.15% 89/275 = 32.36% 4/89 = 4.49%* 186/275 = 67.64%
0 nM = 90 ova 27 = 30.%* 63 = 70.0%
0 nM = 91 ova 25 = 27.47%* 66 = 72.53%

(*) Significantly difference at p < 0.05 compared to the others without star at the same type of treatment or columens.