Table 3.

Characteristics of mutation status in patients with GIST (n = 32).

No.	Amplification		Mutation status
	1st PCR	Nested PCR	Gene	Exon	Codonb, c	Type
1	Failure	〇	None	–	–	–
2	〇		KIT	11	V559D	PM
3	〇		KIT	9	503AY504	I
4	〇		KIT	13	K642E	PM
5	〇		PDGFRA	18	D842V	PM
6	〇		KIT	11	550–558	D
7	〇		KIT	11	555	D
8	〇		KIT	11	W557R	PM
9	〇		KIT	11	579DPTQLPYD580	I
10	〇		KIT	11	W557R	PM
11	〇		PDGFRA	18	D842V	PM
12	〇		KIT	11	580DH581	I
13	〇		KIT	11	V560E	PM
14	〇		KIT	11	573DP574	I
15	〇		KIT	11	585YDHKWEFP586	I
16	〇		KIT	11	V560D	PM
17	〇		KIT	11	L577P	PM
18	〇		KIT	11	V561D	PM
19	〇		KIT	11	L577P	PM
20	〇		KIT	11	V560D	PM
21	〇		KIT	11	L576P	PM
22	〇		KIT	11	561–562	D
23	Failure	〇	KIT	11	560	D
24	〇		KIT	11	V559D	PM
25	〇		KIT	11	558	D
26	〇		KIT	11	580DPTQLPYDH581	I
27	〇		KIT	11	560	D
28	Failure	〇	None	–	–	–
29a	Failure	Failure	None	–	–	–
30	〇		KIT	11	V559D	PM
31	〇		KIT	11	585LPYDHKWEFP586	I
32	〇		KIT	11	557–558	D

^aMutation status of No.29 was analyzed using surgical tissue sample because of failure to amplification by FNA sample.

^bEach letter indicates the amino acid that was inserted or substituted due to the gene mutation (e.g., V559D indicates that valine was substituted for aspartic acid at codon 559 due to a point mutation, and 503AY504 indicates that alanine and tyrosine were inserted between codons 503 and 504).

^cThe numbers in the codon column indicate deleted codons (e.g., 557–558 indicates that codons 557 and 558 were deleted).

Abbreviations. D, deletion; PM, point mutation; I, insertion.