Morphological characters of Tuber luomae (Holotype, OSC 148707). A. Ascocarp, surface and cross-section view. B. Outer peridium in cross section composed of inflated cells with inner peridium abruptly differentiated as interwoven hyphae, stained with Safranin O/ Fast-green. C. One- to four-spored asci. D. Spiny spores stained with Safranin O/ Fast-green. E. Four-spored ascus with distinctive basal hyphal stem. F. Spore ornamentation, highlighting the spines. G. Spores at two focal depths illustrating the spines and lack of connecting reticulum. Scale bars: A = 10 mm; B, D = 20 μm; C = 40 μm; E–G = 15 μm.