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. 2020 Aug 9;3:100061. doi: 10.1016/j.jtauto.2020.100061

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© 2020 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 2 — Developing Clone 5 thymocytes in animals constructed with DQ8⁺ HSCs show phenotypic characteristics of negative selection. Thymocytes were analyzed by FCM. Within each sample the cells are first gated into GFP⁺ and GFP^- populations. All further analysis is conducted on both populations separately, treating the GFP- population as an internal control within each mouse. a. Comparison of the percentage of CCR7⁺ CD4SP in total GFP⁺ and GFP^- thymocytes within the same mouse transplanted with Clone 5 or GFP-only transduced cells. b., c. Representative staining of grafted thymi of mice transplanted with b. DQ8⁺ HSCs c. DQ8^- HSCs. Cells are gated on GFP expression, then on CD4SP prior to analysis of CCR7 expression. n = 3 Clone 5 DQ8^-, n = 4 Clone 5 DQ8⁺, n = 2 GFP-only DQ8^-, n = 3 GFP-only DQ8⁺. ∗ = p < 0.05 by paired t-test. Mice marked with a circle are transgenic for HLA-DQ8.