Fig. 3.
(A) Effects of impaired signaling on lifespan at 20 °C in the sod-1 mutant. Mean lifespan ±SD was as follows; 29.8 ± 7.7 days in the wild-type strain, 25.1 ± 6.8 days in the sod-1 mutant, and 19.9 ± 4.2 days in the sod-1;daf-16 mutant. Mean lifespan ±SD was as follows; 29.9 ± 7.3 days in the wild-type strain, 19.5 ± 17.0 days in the sek-1 mutant, and 19.7 ± 13.6 days in the sod-1;sek-1 mutant. Mean lifespan was different between the sod-1 and sod-1;daf-16 mutants (P < 0.0001), but not between the sek-1 and sod-1;sek-1 mutants. Means were compared by Student's t-test was performed. (B) Real-time PCR data of the sod genes expression in the strains. Data shown represent the means ± SEM. Means were compared by Student's t-test (*P < 0.05, **P < 0.01, ***P < 0.001). (C) O2.- production in mitochondria, cytosol and SMP of the strains. Open, shaded and closed columns indicate O2.- levels of the wild-type strain, sek-1 and sod-1;sek-1 mutants, respectively. Data are shown as means ± SD. Means were compared by Student's t-test (***P < 0.0001).