Fig. 2.

Endochondral ossification was promoted after PR. (A) The represented sections show the metaphysis labelled with alizarin and calcein of wild-type mice. Green and red areas were stained with calcein and alizarin, respectively. The unstained area and green area represent the area grown by 72 and 48 h, respectively. Squared parts are magnified in each lower image. Double arrows indicate the unstained area in the metaphyseal trabecular. Scale bares indicate 300 μm in upper images and 100 μm in lower images. (B) Mean and SD of relative longitudinal length of the unstained area and green area in wild-type mice. The longitudinal length of the unstained area was significantly increased after PR. The statistical differences shown on each graph are analyzed by paired t-test. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)