Figure 2.
Influence of the bs and structural properties of the phase-separated clusters. (A) The interaction between the bs and the binders influences the system properties by locally increasing the concentration around the bs. (B) Fraction of total binders forming the largest cluster Nb/Ntot for different concentrations c and Eb-bs, at the transition energy Eb-b = 2.7 KBT. When c is ∼1.1%, only at Eb-bs = 5.1 KBT does the system exhibits a macroscopic transition. (C) Left panels show distance between single binders and the center of the phase-separated cluster, as function of time, for two different values of Eb-b. Each color represents a different binder. The horizontal dashed line indicates an estimate of the cluster size. When the dot is above the line, the binder is escaped from the cluster. Right panels show zoom highlighting of the mobility of the binders when they move within the cluster. (D) The fraction of binders μ that detach from the cluster at least once in a characteristic time interval. For low affinities, the cluster exhibits highly dynamical properties because up to roughly 70% of the binders experience a detaching event. (E) The attaching time—that is, the average relative time spent by a single binder in the cluster—strongly depends on the binding affinity Eb-b. (F) The mean-square displacement (MSD; log-log scale) of the binders within the cluster, for different values of Eb-b. As the energy decreases, the binders have a higher mobility, and the cluster becomes more dynamic. For (C)–(F), the concentration used is c ∼1.5%. To see this figure in color, go online.