Figure 3.

Multiphoton excited FT-FRAP with comb photobleach of FITC-polydextran (2 MDa) dissolved in 50:50 glycerol/water. (A) Image of the solution immediately after a 32-line comb bleach. (B) 2D FT of (A) with the circled peaks corresponding to the first and second spatial frequency harmonics of the 32-line comb bleach. (C) Fluorescence recovery of the first and second harmonic peaks with best-fit curves recovering a diffusion coefficient (D = 3.70 ± 0.02 μm²/s). The reported uncertainty is the SD of the fit. To see this figure in color, go online.